Application of multiplex nested RT-PCR for fast detection of PDGFRα fusion gene in myeloproliferative neoplasms / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1447-1449, 2011.
Article
in Zh
| WPRIM
| ID: wpr-331057
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore the applicable value of multiplex nested reverse transcription-polymerase chain reaction (multiplex nested RT-PCR)for the detection of platelet-derived growth factor receptor alpha (PDGFRα) fusion gene in myeloproliferative neoplasms (MPN). Bone marrow or peripheral blood samples from 146 patients with MPN were analyzed by using a novel multiplex nested RT-PCR. The result showed that PDGFRα fusion gene was found in 6 out of the 146 bone marrow or peripheral blood samples, the positive rate was 4.11%, 4 from the 6 patients received treatment with imatinib and showed therapeutic effect. It is concluded that the multiplex nested RT-PCR has a series of advantages such as high sensitivity, specificity, and time-saving, and can be applied for determination of the molecular type of MPN, and also for the diagnosis and therapy of MPN.
Full text:
1
Index:
WPRIM
Main subject:
Bone Marrow Neoplasms
/
Reverse Transcriptase Polymerase Chain Reaction
/
Receptor, Platelet-Derived Growth Factor alpha
/
Diagnosis
/
Gene Fusion
/
Multiplex Polymerase Chain Reaction
/
Genetics
/
Methods
/
Myeloproliferative Disorders
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2011
Type:
Article