Characteristics of MSCs adhesion to polypeptides modified surface polymer PLGA-ASP-PEG / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 71-75, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-331392
ABSTRACT
In this study we examined the in vitro characteristics of MSCs adhesion to polypeptides modified surface of polymer PLGA-[ASP-PEG]. We study the adhesion of marrow stromal cells in biomaterials at diferrent times using a micropipette aspiration technique. Comparison the adhesion of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides versus PLGA-[ASP-PEG]. The adhesive conditions of MSCs on the materials were observed by scanning electron microscope. Four hours after MSCs inoculating in biomerials, the cell adhesion force of PLGA-[ASP-PEG] is 172.78 +/- 15. 23N and the force of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides is 209.47 +/- 92.59N. There are no difference between two biomaterials. After 12 hours,the adhesion force of PLGA-[ASP-PEG] combinating GRGDSPC polypeptides is 576.23 +/- 165.74N, and the cell force of PLGA-[ASP-PEG] is 261.84 +/- 100.09 N. There are very significant difference between the two biomaterials. However, after 24 hours,the adhesion forces of the two biomaterials have no difference. The density of MSCs on PLGA-[ASP-PEG]-GRGDSPC surface was much higher than that of PLGA-[ASP-PEG]. Combination polypeptides in the surface of biomaterials can enhance the adhesion of MSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Polyglycolic Acid
/
Biocompatible Materials
/
Bone Marrow Cells
/
Cell Adhesion
/
Cells, Cultured
/
Chemistry
/
Rats, Sprague-Dawley
/
Aspartic Acid
/
Lactic Acid
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2007
Type:
Article
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