Experiment treatment of collagen-induced arthritis in rats with recombinant plasmid containing vasoactive intestinal peptide gene / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 403-410, 2006.
Article
in Zh
| WPRIM
| ID: wpr-332134
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutical effect of recombinant plasmid containing vasoactive intestinal peptide gene (pcDNA3.1+/VIP) on collagen-induced arthritis (CIA) in rats.</p><p><b>METHODS</b>The experimental arthritis was induced by intradermal injection of bovine type II collagen emulsified in Freund's adjuvants in male SD rats. The rats then were given intra-articular injection with recombinant plasmid (pcDNA3.1+/VIP). The levels of serum TNF-alpha, IL-4 and IL-2 were detected by Avidin-Biotin Peroxdase Complex-enzyme-linked immunosorbent assay (ABC-ELISA) and the pathological changes in the joint of rats were observed.</p><p><b>RESULT</b>Histological examination showed massive inflammatory infiltration in the joint with destruction of bone and cartilage, while the severity of pathological changes in synovia of VIP-treated rats was markedly reduced. Compared with normal group, the serum TNF-alpha, IL-2 levels of CIA rats were significantly increased (P <0.05) and IL-4 level was decreased (P<0.05). Compared with control and pcDNA3.1+ -treated CIA rats, serum TNF-alpha and IL-2 levels of pcDNA3.1+/VIP-treated rats were decreased and IL-4 level was increased (P<0.05).</p><p><b>CONCLUSION</b>Recombinant plasmid containing vasoactive intestinal peptide gene (pcDNA3.1+/VIP) can reduce the clinical and histological severity of established CIA and it might be a promising candidate for treatment of rheumatoid arthritis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Arthritis, Experimental
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Arthritis, Rheumatoid
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Therapeutics
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Recombinant Proteins
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Vasoactive Intestinal Peptide
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Genetic Therapy
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Random Allocation
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Rats, Sprague-Dawley
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Therapeutic Uses
Limits:
Animals
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article