Effects of lipid rafts on signal transmembrane transduction mediated by c-Met / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 449-452, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332207
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of lipid rafts on cell signal transmembrane transduction mediated by c-Met.</p><p><b>METHODS</b>After HepG2Cells were treated with MbCD to disrupt the lipid rafts and were treated with artificial recombination hepatocyte growth factor to activate c-Met, the activities of PLCr1/PKC, PI3K/Akt and MAPK signaling pathways in HepG2 cells were analyzed using Western blot.</p><p><b>RESULTS</b>(1) After disruption of lipid rafts with MbCD, phosphorylation of PLCr1 decreased by 35% (P = 0.022); the content of PLCr in the cytoplasm increased by 1.75 fold (P = 0.017); PLCr1 conjugated with membrane decreased by 30% (P = 0.037). (2) The content of PKB in the cytosol decreased by 38% (P = 0.028), and the phosphorylation level of PKB conjugated with membrane decreased by 14% (P = 0.041). At the same time, PDK translocation from cytosol to the plasma membrane and its activation were inhibited by treatment with MbCD. (3) Treatment with MbCD had no significant effect on ErK/MAPK, p38/MAPK and JNK/MAPK signaling pathways.</p><p><b>CONCLUSION</b>Disruption of lipid rafts with MbCD inhibits the activation of PLCr1/PKC and PI3K/PKB signaling pathways by HGF/cMet, but has no effect on MAPK signaling pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phosphorylation
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Signal Transduction
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Phosphatidylinositol 3-Kinases
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Proto-Oncogene Proteins c-met
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Mitogen-Activated Protein Kinases
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Membrane Microdomains
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Phospholipase C gamma
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Proto-Oncogene Proteins c-akt
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Hep G2 Cells
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2008
Type:
Article