Inhibition of vegfr-2 gene expression by RNA interference / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1260-1264, 2010.
Article
in Zh
| WPRIM
| ID: wpr-332381
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore a novel potential gene target for therapy of malignant tumor. The recombinant expression plasmids of VEGF/VEGFR-2 were designed, constructed and then transfected into A549 cells by using lipofectamine. The expressions of VEGF/VEGFR-2 mRNA and protein were detected by RT-PCR and Western blot respectively. The cell proliferation was assayed by CCK-8 and the cell apoptosis was detected using Hoechst Staining. The results indicated that as compared with the blank control, pGenesil-1 and scramble groups, the interference effect of pGenesil-1-vegfr-2-shRNA-1 vector group was more obvious. As the expression of endogenous vegfr-2 mRNA decreased, the expression of VEGFR-2 protein decreased correspondingly. The proliferation of A549 cells was inhibited significantly by RNAi at 72 hours (p<0.01). The apoptosis of A549 cells was induced at 48 hours after being transfected with pGenesil-1-vegfr-2-shRNA-1 and the typical apoptosis morphology could be seen by fluorescence microscopy. It is concluded that the expression of vegfr-2 gene is inhibited effectively by vegfr-2 specific shRNA. The proliferation of A549 cells is inhibited significantly and the apoptosis of cells is induced. This result showed that VEGF/VEGFR-2 signaling pathway can be an effective target for the prevention and treatment of malignant tumor.
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Transfection
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Gene Expression
/
Apoptosis
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Vascular Endothelial Growth Factor Receptor-2
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RNA, Small Interfering
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RNA Interference
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Cell Line, Tumor
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Cell Proliferation
/
Genetic Vectors
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article