Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 488-491, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332457
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.</p><p><b>METHODS</b>effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.</p><p><b>RESULTS</b>all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.</p><p><b>CONCLUSION</b>ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.</p>
Full text:
1
Index:
WPRIM
Main subject:
Virus Cultivation
/
Biotechnology
/
Enzyme-Linked Immunosorbent Assay
/
Viral Vaccines
/
Vaccines, Inactivated
/
Cells, Cultured
/
Sensitivity and Specificity
/
Cell Culture Techniques
/
Reverse Transcriptase Polymerase Chain Reaction
/
Hepatitis A Vaccines
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article