Protection effects of acetylcholinesterase inhibitors on apoptosis / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 458-460, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332467
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether tacrine and donepezil can prevent apoptosis induced by Lipopolysaccharides.</p><p><b>METHODS</b>Phase-contrast microscopes was used to observe the morphological changes of Vero cells. Cell counting kit-8 was used to measure cell survival vitro. DNA fragment was analyzed by agarose gel electrophoresis to determine the apoptotic biochemical changes.</p><p><b>RESULTS</b>Vero cells treated with 400 microg/ml and 500 microg/ml Lipopolysaccharides exhibited cell apoptosis. 10 micromol/L tacrine provided protective effect to 500 microg/ml Lipopolysaccharides induced cell apoptosis measured by Phase-contrast microscopes, cell counting kit-8 and DNA fragment analyze. However, donepezil did not show any protective effect of the apoptosis induced by 500 microg/ml Lipopolysaccharides.</p><p><b>CONCLUSION</b>Lipopolysaccharides can induce apoptosis in Vero cells to built an apoptotic model in vitro. Tacrine rather than donepezil can inhibit Lipopolysaccharides induced apoptosis in Vero cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Piperidines
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Tacrine
/
Vero Cells
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HeLa Cells
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Cell Count
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Cell Survival
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Chlorocebus aethiops
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article