A cell-based detection of ciguatoxin using sodium fluorescence probe / 南方医科大学学报
Journal of Southern Medical University
; (12): 653-655, 2011.
Article
in Zh
| WPRIM
| ID: wpr-332515
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a cell-based detection method of ciguatoxin using fluorescence assay.</p><p><b>METHODS</b>Mouse neuroblastoma N-2A cells were exposed to ouabain and veratridine and different concentrations of standard ciguatoxin samples (P-CTX-1) to establish the curvilinear relationship between the toxin dosage and fluorescence intensity using the sodium fluorescence probe CoroNaTM Green. The toxicity curvilinear relationship was also generated between the toxin dosage and cell survival using CCK-8 method. Based on these standard curves, the presence of ciguatoxin was detected in 33 samples of deep-sea coral fish.</p><p><b>RESULTS</b>A correlation was found between the detection results of cell-based fluorescence assay and cytotoxicity assay, whose detection limit reached 103 g/ml and 1012 g/ml, respectively. The cell-based fluorescent assay sensitivity showed a higher sensitivity than cytotoxicity assay with a 2-4 h reduction of the detection time.</p><p><b>CONCLUSIONS</b>The cell-based fluorescent assay can quickly and sensitively detect ciguatoxin and may serve as a good option for preliminary screening of the toxin.</p>
Full text:
1
Index:
WPRIM
Main subject:
Sodium
/
Cytotoxicity Tests, Immunologic
/
Ciguatoxins
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Cell Line, Tumor
/
Toxicity
/
Fishes
/
Fluorescent Dyes
/
Methods
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article