Effects of Different Culture Systems on the Hematopoietic Differentiation Ability of iPS Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1863-1868, 2016.
Article
in Zh
| WPRIM
| ID: wpr-332596
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the in vitro effects of different culture systems on hematopoietic differentiation ability of induced pluripotent stem (iPS) cells.</p><p><b>METHOD</b>Two culture systems including E8 and mTESR(freeder-free medium), and the classical ES culture medium were chosen for culture of iPS cells. The iPS cells maintaining in above mentioning culcure systems were co-cultured with OP9 cells(murine bone marrow stromal cells) in vitro to be induced to differentiate into hematopoietic stem/progenitor cells. Flow cytometry and real-time quantitative PCR were used to detect the expression of specific hematopoietic markers and the effects of different culture systems on the differentiation of iPS in vitro.</p><p><b>RESULT</b>iPS cultured in the 3 selected medium could be differentiated into hematopoietic stem cells. Efficiency of hematopoietic differentiation was up to 28.4% in classical ES culture system, which was significantly higher than that in E8 and mTESR system.</p><p><b>CONCLUSION</b>Under the co-culture with OP9, iPS can differentiate into hematopoietic stem/progenitor cells, which shows higher efficiency when iPS maintained in the ES medium.</p>
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article