Detection of weak D antigen by flow cytometry / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 474-477, 2013.
Article
in Zh
| WPRIM
| ID: wpr-332755
Responsible library:
WPRO
ABSTRACT
Flow cytometry was previously applied for analysis of Rh(D) antigen density, therefore it was suggested that the flow cytometry may be used for routine detection of weak D positive phenotypes. This study was purposed to evaluate its practicability. Six weak D positive and 7 DEL individuals were detected by using saline, IAT and absorption/elution test from 2010 to 2011 years. By RHD genotyping, zygosity analysis and sequencing, 3 cases of weak D type 15, 3 cases of partial D type DVI-III and 7 cases of DEL carrying RHD1227A alleles were identified. Taking 2 normal Rh(D)-positive and 2 D-negative samples as controls, all the samples were tested by using flow cytometry, and the median fluorescence intensities were observed as well. The results indicated that all weak D type 15 and partial D type DVI samples were detected to be positive by flow cytometry, as compared with 2 Rh(D)-negative samples (P < 0.05). Seven 7 DEL samples were tested to be negative (P > 0.05), although one of 7 DEL was tested as "±" in IAT and strong positive in absorption/elution. The RHD zygosity analysis showed this DEL individual as RHD(+)/RHD(+) homozygote. It is concluded that the sensitivity of detecting D antigen by flow cytometry is similar to that of IAT, but lower than absorption/elution test. As for detecting weak D or partial D antigens, IAT is easier than flow cytometry; as for identifying DEL, the flow cytometry is not sensitive enough.
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
Rh-Hr Blood-Group System
/
Blood
/
Blood Donors
/
Blood Grouping and Crossmatching
/
Alleles
/
Allergy and Immunology
/
Flow Cytometry
/
Genetics
/
Genotype
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adult
/
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article