Protective effect of Sanhuangyinchi Fang drug serum on hydrogen peroxide-induced DNA oxidative damage in LO2 cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1434-1439, 2015.
Article
in Zh
| WPRIM
| ID: wpr-333609
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the protective effect of Sanhuangyinchi Fang drug serum (SF) against hydrogen peroxide-mediated DNA oxidative damage in LO2 cells.</p><p><b>METHODS</b>The LO2 cells were randomly divided into the control group, H(2)O(2) group, SF groups (5%, 10%, and 15%) and vitE group. The morphological features of the treated LO2 cells were observed under inverted microscope. The viability of the treated cells was assessed with CCK-8 method, and the activity of SOD, CAT and GSH-PX were detected biochemically. Reactive oxygen species (ROS) levels, the content of 8-OHdG, and DNA damage of the cells were evaluated by flow cytometry, ELISA, and Comet assay, respectively.</p><p><b>RESULTS</b>Compared with H(2)O(2) group, the cells in SF groups (10% and 15%) and vitE group showed higher cell survival rate (P<0.05) and higher SOD, CAT, GSH-PX (P<0.05) and ROS scavenging activities (P<0.01) with markedly decreases the content of 8-OHdG (P<0.01) and reduced tailing ratio, tail length, tail moment and Olive tail moment (P<0.05).</p><p><b>CONCLUSION</b>SF drug serum, especially at the concentration of 15%, can protect LO2 cells from H(2)O(2)-mediated DNA oxidative damage.</p>
Full text:
1
Index:
WPRIM
Main subject:
Oxidation-Reduction
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Pharmacology
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DNA Damage
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Drugs, Chinese Herbal
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Cell Line
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Reactive Oxygen Species
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Oxidative Stress
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Protective Agents
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Comet Assay
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Deoxyguanosine
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article