Nuclear factor I-C inhibits platelet-derived growth factor-induced enhancement of dermal fibroblast sensitivity to TGF-β / 南方医科大学学报
Journal of Southern Medical University
; (12): 1245-1250, 2015.
Article
in Zh
| WPRIM
| ID: wpr-333647
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of nuclear factor I-C (NFI-C) on platelet-derived growth factor (PDGF)-induced up-regulation of TGF-β receptor II (TβRII) in dermal fibroblasts.</p><p><b>METHODS</b>A lentiviral vector containing NFI-C sequence (Lenti-GFP-NFI-C) was transfected into a human foreskin fibroblast cell line (HFF-1). Cultured HFF-1 cells, cells transfected with Lenti-GFP-NFI-C, and cells transfected with a negative virus were stimulated with PDGF-BB, and Western blotting and RT-qPCR were used to detect the expression levels of TβRII in the treated cells.</p><p><b>RESULTS</b>PDGF treatment significantly increased the expression level of TβRII in HFF-1 cells (P<0.05). The cells transfected with Lenti-GFP-NFI-C expressed a significantly lower level of TβRII than non-transfected cells in response to PDGF stimulation (P<0.05), but the negative virus showed no such inhibitory effect (P>0.05). No significant difference was found in the expression level of TβRII protein between cells transfected with Lenti-GFP-NFI-C-transfection before PDGF stimulation and the blank control cells.</p><p><b>CONCLUSION</b>NFI-C can inhibit PDGF-induced up-regulation of TβRII and thus reduce the sensitivity of the dermal fibroblasts to TGF-β.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Platelet-Derived Growth Factor
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Transfection
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Up-Regulation
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Cell Line
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Transforming Growth Factor beta
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Protein Serine-Threonine Kinases
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Receptors, Transforming Growth Factor beta
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Lentivirus
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Proto-Oncogene Proteins c-sis
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article