Study of loss of heterozygosity in oligodendroglial tumors by real-time quantitative polymerase chain reaction-based microsatellite analysis / 中华病理学杂志
Chinese Journal of Pathology
; (12): 731-734, 2006.
Article
in Zh
| WPRIM
| ID: wpr-333983
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the loss of heterozygosity (LOH) at chromosomes 1p or 19q in oligodendroglial tumors.</p><p><b>METHODS</b>Twenty-eight cases of oligodendroglial tumors were enrolled into the study. Real-time quantitative polymerase chain reaction-based microsatellite analysis was performed on paraffin-embedded tumor tissues in order to study the status of chromosomes 1p and 19q.</p><p><b>RESULTS</b>Among the 28 cases of oligodendroglial tumors, 24 cases (85.7%) showed 1p LOH, while 18 cases (64.3%) showed 19q LOH and 17 cases (60.7%) showed LOH of both 1p and 19q. LOH at 1p or 19q was present in 25 (89.3%) of the 28 cases.</p><p><b>CONCLUSIONS</b>Real-time quantitative polymerase chain reaction-based microsatellite analysis is a rapid and specific way in detecting LOH in paraffin-embedded tumor tissues. LOH at 1p or 19q is present in majority of the oligodendroglial tumors studied.</p>
Full text:
1
Index:
WPRIM
Main subject:
Oligodendroglioma
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Chromosomes, Human, Pair 1
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Chromosomes, Human, Pair 19
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Brain Neoplasms
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Polymerase Chain Reaction
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Microsatellite Repeats
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Loss of Heterozygosity
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Genetics
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Methods
Limits:
Adult
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article