Cloning and expression of single-chain Fv antibodies against H5N1 Avian influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
; (6): 63-67, 2009.
Article
in Zh
| WPRIM
| ID: wpr-334761
Responsible library:
WPRO
ABSTRACT
To construct Fv antibodies against H5N1 Avian influenza virus hemagglutinin,extracted mRNA from B lymphoblastoid cell lines secreting anti-HA antibodies was used and the VH and VL genes were amplified by RT-PCR and linked together by splicing overlap extension (SOE) with (Gly4 Ser)3 linker. The recombinant plasmid was then transformed to E. coli BL21(DE3) and sequence analysis indicated the total length of scFv was 714 bp and the expression of Fv was validated by PAGE and Western blot.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Viral Proteins
/
Virology
/
Birds
/
Recombinant Fusion Proteins
/
Immunoglobulin Variable Region
/
Gene Expression Regulation
/
Cloning, Molecular
/
Immunoglobulin Heavy Chains
/
Immunoglobulin Light Chains
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2009
Type:
Article