The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2 / 病毒学报
Chinese Journal of Virology
; (6): 83-87, 2008.
Article
in Zh
| WPRIM
| ID: wpr-334842
Responsible library:
WPRO
ABSTRACT
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
Full text:
1
Index:
WPRIM
Main subject:
Viral Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Hepatitis Antibodies
/
Hepatitis E virus
/
Allergy and Immunology
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
/
Epitopes
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2008
Type:
Article