Identification of a functional ITAM-like sequence within G1 cytoplasmic tail of Hantaan virus / 病毒学报
Chinese Journal of Virology
;
(6): 424-428, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-334871
ABSTRACT
The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Physiology
/
Protein-Tyrosine Kinases
/
Molecular Sequence Data
/
Signal Transduction
/
Cells, Cultured
/
Chemistry
/
Viral Envelope Proteins
/
Amino Acid Sequence
/
Hantaan virus
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2007
Type:
Article
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