Enhancing ethanol production using thermophilic yeast by response surface methodology / 生物工程学报
Chinese Journal of Biotechnology
; (12): 42-47, 2010.
Article
in Zh
| WPRIM
| ID: wpr-336263
Responsible library:
WPRO
ABSTRACT
We optimized the conditions of simultaneous saccharification and fermentation (SSF) from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10. Based on the single factor experiment, we screened the important parameters by Plackeet-burman design. We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. Then, we obtained the optimum values of the parameters by Box-Behnken design. The results showed that the important parameters were the liquefaction time, glucosidase dosages and initial concentration of cassava flour (substrate). The optimum technical conditions were as follows: liquefaction time 35 min, glucosidase dosages 1.21 AGU/g substrate and initial substrate concentration 37.62%. Under such optimum conditions, the ethanol yield of 20 L fermentor reached 16.07% (V/W) after 48 h fermentation at 37 degrees C and 100 r/min. The ethanol content increased 33% than that under the original condition.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Substrate Specificity
/
Yeasts
/
Manihot
/
Ethanol
/
Fermentation
/
Glucosidases
/
Hot Temperature
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2010
Type:
Article