Construction and characterization of an attenuated recombinant Listeria monocytogenes vector vaccine delivering HPV16 E7 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 683-692, 2016.
Article
in Zh
| WPRIM
| ID: wpr-337431
Responsible library:
WPRO
ABSTRACT
Listeria monocytogenes (L. monocytogenes, LM) is an excellent tumor vaccine vector. In this study, recombinant LM vaccine candidate expressing human papillomavirus type 16 (HPV16) E7 protein was constructed and its charactericts were determined. Through homologous recombination, E7 gene was cloned in frame with the LM4 Phly promoter-signal sequence, and introduced into the chromosome of LM4. The recombinant strain named LM4△hlyE7 with the plasmid-free and antibiotic-resistant gene-free was constructed. LM4△hlyE7 could express and secrete E7-LLO fusion protein; its size is 66 kDa and has immunological activity. Furthermore, LM4△hlyE7 could multiply in RAW264.7 macrophages by confocal laser scanning microscope. Additionally, LM4△hlyE7 could induce specific antibodies against E7 in immunized mice in ELISA. Also, the 50% lethal dose (LD₅₀) of LM4△hlyE7 strain was 3.863×10⁹ CFU (Colony-Forming Units) in C57BL/6 mice with intraperitoneal immunization, which was more attenuated than wild type LM4. Mice immunized with LM4△hlyE7 did not show obvious pathological change. These data show that LM4△hlyE7 expressing E7-LLO fusion protein has good safety, which may provide the materials for research of antitumor effect and would be a promising vaccine candidate for cervical cancer.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Recombinant Fusion Proteins
/
Vaccines, Attenuated
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Viral Vaccines
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Cancer Vaccines
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Papillomavirus Infections
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Allergy and Immunology
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Papillomavirus E7 Proteins
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Listeria monocytogenes
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Mice, Inbred C57BL
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2016
Type:
Article