Study on expression of cytokines mRNA induced by B7-1-transfected Raji and Jurkat cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 322-326, 2002.
Article
in Zh
| WPRIM
| ID: wpr-337629
Responsible library:
WPRO
ABSTRACT
To investigate the function of B7 co-stimulator in activation and differentiation of T cell, B7 gene was transfected into Raji and Jurkat cells by liposome, B7 expression in tumor cells was detected with flow cytometry, and expression of IL-2, IL-4 and IFN-gammamRNA was detected by RT-PCR. Kinetics of secretion of three cytokines was also analyzed at 4, 12, 20 and 48 hours after gene transfection. The results showed that B7(+) Raji cells could induce mRNA expression of IL-2, IL-4 and IFN-gamma on T cell surface; B7(+) Jurkat cells could induce secretion of IL-2 and IFN-gamma. However, B7(-) Raji and B7(-) Jurkat cells could not induce secretion of cytokines. Kinetics of the three cytokines secretion were different, IL-2 and IL-4 were only detectable after 4 hours of T cell activation, whereas IFN-c was detectable after 12 hours of stimulation. The peak levels of IL-2, IL-4 and IFN-gamma were found at 20 hours after activation. It was concluded that tumor cell lines transfected with B7 gene could enhance their immunocompetence, activating T cell efficiently and B7-1 play more critical role in T cell activation and differentiation.
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
RNA, Messenger
/
Transfection
/
Cytokines
/
Interleukin-4
/
Interferon-gamma
/
Interleukin-2
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B7-1 Antigen
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Jurkat Cells
/
Genetics
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2002
Type:
Article