Azoospermia factor microdeletions in idiopathic azoospermia and severe oligozoospermia / 中华男科学杂志
National Journal of Andrology
; (12): 108-111, 2006.
Article
in Zh
| WPRIM
| ID: wpr-338355
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the relationship between microdeletions of AZF( azoospermia factor) on Y chromosome in male with idiopathic azoospermia and severe oligozoospermia.</p><p><b>METHODS</b>Only patients with an apparently normal 46,XY karyotype and normal FSH, LH and T were included in this study. Multiplex PCR was used to detect the sequence-tagged sites( STS) as follows :sY84, sY86, sY127, sY134, sY152, sY153, sY254, sY255, and ZFX/Y was used as internal control gene.</p><p><b>RESULTS</b>No microdeletion was detected in the control whereas 8 microdeletion cases existed in 67 idiopathic azoospermia and severe oligozoospermia, including 4 in AZFc, 2 in AZFa + AZFc, 1 in AZFc + AZFb, and 1 in AZFb. The prevalence rate of microdeletion was 11.94%, which was statistically different from the control.</p><p><b>CONCLUSION</b>Microdeletions in the AZF regions on the long arm of the Y-chromosome are associated with idiopathic azoospermic and severely oligozoospermic men. Multiplex PCR was a rapid and reliable method for screening microdeletions of AZF.</p>
Full text:
1
Index:
WPRIM
Main subject:
Oligospermia
/
Polymerase Chain Reaction
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Chromosome Deletion
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Seminal Plasma Proteins
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Chromosomes, Human, Y
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Azoospermia
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Genetic Loci
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Genetics
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Karyotyping
Limits:
Adult
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Humans
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Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2006
Type:
Article