Effect of Shenfu injection on nuclear factor-kappaB during myocardial ischemia/reperfusion injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 200-204, 2005.
Article
in En
| WPRIM
| ID: wpr-338613
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of Shenfu injection on the concentrations of plasma tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), activity of Nuclear Factor kappa B (NF-kappaB) and heart tissue ultrastructure during myocardial ischemia/reperfusion (I/R) injury in rats and its potential mechanism.</p><p><b>METHODS</b>Myocardial ischemia/reperfusion (I/R) was produced by ligation and release of the left anterior descending coronary artery. Ischemia lasted for 30 min and reperfusion for 60 min. Twenty-four healthy male SD rats weighing 230-280 g were randomly divided into three groups (n = 8, each): Group I (Sham-operation group); Group II (I/R group); Group III (Shenfu group), in which Shenfu injection (10 ml/kg) was intraperitoneally injected 30 min before ischemia in animals with I/R. The plasma concentrations of IL-6 and TNF-alpha were measured by ELISA, and the heart was harvested for determination of NF-kappaB levels by Ecl-western blot analysis. Electron microscopy was used to study its ultrastructure.</p><p><b>RESULTS</b>After reperfusion, NF-kappaB binding activity in myocardial nuclei and the plasma concentrations of IL-6 and TNF-alpha were significantly increased in Group II, compared with Group I (P < 0.01), and they were markedly reduced in Group III, compared with Group II (P < 0.01). In addition, electron microscopic examination showed more serious injury of the myocardium ultrastructure in Group II, while in Group III the myocardial ultrastructure was similar to normal state.</p><p><b>CONCLUSIONS</b>Shenfu injection inhibits NF-kappaB activity in I/R myocardium and leads to down-regulation of proinflammatory cytokine expression, which might be one of the molecular mechanisms of Shenfu injection in cardioprotection.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
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Blood
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Drugs, Chinese Herbal
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Myocardial Reperfusion Injury
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NF-kappa B
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Rats, Sprague-Dawley
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Drug Therapy
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Traumatology
Year:
2005
Type:
Article