A double suicide gene system driven by KDR promoter selectively kills human hepatic carcinoma cells and human umbilical vein endothelial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1-4, 2009.
Article
in Zh
| WPRIM
| ID: wpr-339082
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the selective killing effects of adenovirus (Ad)-mediated double suicide gene system driven by KDR promoter (KDR-CdglyTK) on the human hepatic carcinoma cells and human umbilical vein endothelial cells (HUVECs).</p><p><b>METHODS</b>KDR-expressing BEL-7402 and HUVECs and HepG2 cells that did not express KDR were infected by KDR-CdglyTK, and the infection efficiency and the expression of CdglyTK in the cells was detected by RT-PCR. The infected cells were treated with the the prodrugs 5-FC and GCV at different concentrations, and the cell-killing effects and bystander effects were evaluated by MTT method.</p><p><b>RESULTS</b>At the multiplicity of infection (MOI) of 100, the recombinant AdKDR-CDglyTK showed similar infection efficiency in the 3 cell lines. RT-PCR demonstrated CDglyTK expression in the recombinant adenovirus and the 3 infected cell lines. BEL-7402 and HUVECs infected by the KDR-CdglyTK, but not the HepG2 cells, were highly sensitive to the prodrugs (P<0.001). Bystander effects of the double suicide gene system were observed in the coculture of the infected and non-infected BEL-7402 and HUVECs.</p><p><b>CONCLUSION</b>The double suicide gene system driven by KDR promoter has specific killing effect on KDR-expressing hepatocellular carcinoma cells and HUVECs.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Thymidine Kinase
/
Umbilical Veins
/
Recombinant Fusion Proteins
/
Tumor Cells, Cultured
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Genetic Therapy
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Cells, Cultured
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Adenoviridae
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Promoter Regions, Genetic
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Apoptosis
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article