In vitro study of directional inducible differentiation of inner ear hair cells from human adipose-derived mesenchymal stem cells / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 323-328, 2009.
Article
in Zh
| WPRIM
| ID: wpr-339207
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of directionally inducing human adipose-derived mesenchymal stem cells (hAD-MSCs) towards inner ear hair cells.</p><p><b>METHODS</b>Mesenchymal stem cells from human adipose tissue were isolated, purified and cultured in vitro. hAD-MSCs were induced to neural stem/progenitor-like cell, and then co-cultured with embryonic chick otic vesicle cells. Processed hAD-MSCs were tested by immunostaining to ascertain whether they expressed characteristic hair cell markers.</p><p><b>RESULTS</b>Morphologically, hAD-MSCs were induced to differentiate into neural stem/progenitor cells and expressed specific neural markers. After being co-cultured with embryonic chick otic vesicle cells, hAD-MSCs expressed specific surface markers of inner ear hair cells.</p><p><b>CONCLUSIONS</b>hAD-MSCs can be directionally induced to differentiate towards hair cell-like cells in vitro.</p>
Full text:
1
Index:
WPRIM
Main subject:
Cell Differentiation
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Cells, Cultured
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Adipose Tissue
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Coculture Techniques
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Cell Culture Techniques
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Cell Biology
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Mesenchymal Stem Cells
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Hair Cells, Auditory, Inner
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Methods
Limits:
Animals
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Humans
Language:
Zh
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2009
Type:
Article