Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis virus / 病毒学报
Chinese Journal of Virology
; (6): 12-16, 2013.
Article
in Zh
| WPRIM
| ID: wpr-339981
Responsible library:
WPRO
ABSTRACT
A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease.
Full text:
1
Index:
WPRIM
Main subject:
Reoviridae
/
Bombyx
/
Virology
/
Real-Time Polymerase Chain Reaction
/
Fluorescence
/
Genetics
/
Methods
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2013
Type:
Article