Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells / 病毒学报
Chinese Journal of Virology
; (6): 601-608, 2012.
Article
in Zh
| WPRIM
| ID: wpr-339998
Responsible library:
WPRO
ABSTRACT
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Protein Binding
/
Virology
/
Molecular Sequence Data
/
Cell Line
/
Chemistry
/
Sequence Alignment
/
Amino Acid Sequence
/
Cloning, Molecular
/
Nucleopolyhedroviruses
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2012
Type:
Article