Hypermethylation of the p15(INK4B) gene in acute leukemia and myelodysplastic syndromes / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 987-990, 2002.
Article
in En
| WPRIM
| ID: wpr-340403
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the methylation pattern of the p15(INK4B) gene and to explore its significance in the pathogenesis of acute leukemia (AL) and leukemic transformation of myelodysplastic syndromes (MDS).</p><p><b>METHODS</b>A total of 49 AL cases and 22 MDS cases were analyzed by methylation specific polymerase chain reaction (MSP) for methylation patterns in CpG islands of the p15(INK4B) gene.</p><p><b>RESULTS</b>Hypermethylation of the p15(INK4B) gene was found in 90% (26/29) of newly diagnosed AL, including 46% with complete methylation and 54% with partial methylation. All 3 evolved AL from MDS and 9 relapsed AL showed a methylated p15(INK4B) gene and the proportion of complete methylation was 67% and 56% respectively. Only 5 of 11 (45%) AL in remission, including 2 in complete remission (CR) and 3 in partial remission (PR), were partially methylated. The frequency of p15(INK4B) gene methylation in newly diagnosed or relapsed AL was significantly higher than that in AL in the remission stage (P = 0.002) p15(INK4B) gene methylation was found in 5 of 13 (38%) low-risk MDS (RA/RAS) patients and 80% of them showed only partial methylation. However, p15(INK4B) gene methylation was found in all 9 cases in the high-risk group (RAEB/RAEB-T), including complete methylation in 56%, significantly different from the low-risk MDS group (P = 0.002).</p><p><b>CONCLUSIONS</b>Hypermethylation of the p15(INK4B) gene occurs frequently in leukemia and high-risk MDS. It is possible that hypermethylation of this gene is related to the pathogenesis and development of AL and MDS. It may be used as a gene marker to detect minimal residual disease, relapse of AL and leukemic transformation in MDS.</p>
Full text:
1
Index:
WPRIM
Main subject:
Myelodysplastic Syndromes
/
Leukemia, Myeloid, Acute
/
Cell Cycle Proteins
/
DNA Methylation
/
Cyclin-Dependent Kinase Inhibitor p16
/
Tumor Suppressor Proteins
/
Cyclin-Dependent Kinase Inhibitor p15
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Genetics
Limits:
Humans
Language:
En
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article