The relationship between histone acetylation modification and IFN-gamma responsive gene regulation / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 525-528, 2006.
Article
in Zh
| WPRIM
| ID: wpr-341318
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To study the role of histone modification in the regulation of IFN-gamma-activated gene using chromatin immunoprecipitation technique.</p><p><b>METHODS</b>Real-time PCR was used to measure the mRNA level of interferon-gamma-inducible protein 10 (IP-10) in Hela cells. Chromatin immunoprecipitation combined with Real-time PCR was used to check the histone H4 acetylation level at IFN-stimulated response element (ISRE) locus of IP-10 gene.</p><p><b>RESULTS</b>IP-10 was strongly activated by IFN-gamma. The histone H4 deacetylation happened at the ISRE locus when IP-10 was induced by IFN-gamma. The activation of IP-10 and the deacetylation of histone H4 at the ISRE site induced by IFN-gamma were inhibited or blocked by histone deacetylase (HDAC) inhibitor trichostatin A (TSA).</p><p><b>CONCLUSION</b>The histone H4 deacetylation at the ISRE site is related with the activation of IP-10 by IFN-gamma.</p>
Full text:
1
Index:
WPRIM
Main subject:
Acetylation
/
RNA, Messenger
/
HeLa Cells
/
Histones
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Gene Expression Regulation
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Interferon-gamma
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Chemokine CXCL10
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Genetics
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Histone Deacetylases
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2006
Type:
Article