Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells
Journal of Korean Medical Science
; : 836-842, 2016.
Article
in En
| WPRIM
| ID: wpr-34240
Responsible library:
WPRO
ABSTRACT
To investigate the function and regulation mechanism of ATP-binding cassette, subfamily G, member 2 (ABCG2) in retinoblastoma cancer stem cells (RCSCs), a long-term culture of RCSCs from WERI-Rb1 cell line was successfully established based on the high expression level of ABCG2 on the surface of RCSCs. To further explore the molecular mechanism of ABCG2 on RCSCs, a microRNA that specifically targets ABCG2 was predicted. Subsequently, miR-3163 was selected and confirmed as the ABCG2-regulating microRNA. Overexpression of miR-3163 led to a significant decrease in ABCG2 expression. Additionally, ABCG2 loss-of-function induced anti-proliferation and apoptosis-promoting functions in RCSCs, and multidrug resistance to cisplatin, carboplatin, vincristine, doxorubicin, and etoposide was greatly improved in these cells. Our data suggest that miR-3163 has a significant impact on ABCG2 expression and can influence proliferation, apoptosis, and drug resistance in RCSCs. This work may provide new therapeutic targets for retinoblastoma.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Retinoblastoma
/
Neoplastic Stem Cells
/
Base Sequence
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Transfection
/
Sequence Alignment
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Apoptosis
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Drug Resistance, Neoplasm
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3' Untranslated Regions
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Gene Silencing
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MicroRNAs
Limits:
Humans
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2016
Type:
Article