Freeze-drying of oxygen-carrying substances and storage stability of the freeze-dried solids / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 678-693, 2008.
Article
in Zh
| WPRIM
| ID: wpr-342766
Responsible library:
WPRO
ABSTRACT
Oxygen-carrying substances based on poly-placental hemoglobin were put into freeze-drying. Sucrose was chosen to inhibit the methmoglobin(MetHb) formation. MetHb content, ultravioletes(UV) spectrum, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), High performance liquid chromatography (HPLC) were monitored during freeze-drying. As a result, when the mass ratio of sucrose to protein was above 0.5, MetHb formation was under control;the characteristic absorption of UV spectrum, SDS-PAGE and HPLC showed no visible change. Freeze-dried solids were kept under room temperature and refrigerator for 3 months. As a result, MetHb formation depended upon storage temperature and the mass ratio of sucrose to protein. For group C in which the mass ratio of sucrose to protein was 1.0; there is no marked change in MetHb content, UV spectrum, SDS-PAGE and HPLC after 3 months.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Placenta
/
Sucrose
/
Hemoglobins
/
Methemoglobin
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Drug Stability
/
Drug Storage
/
Electrophoresis, Polyacrylamide Gel
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2008
Type:
Article