Phage resistance of Corynebacterium crenatum conferred by the restriction and modification system cglI / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 760-765, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-342839
ABSTRACT
In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PCR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cglI into C. crenatum. Results showed that the recombinant strains possessed strong phage-resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contamination in amino acid fermentation industry.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Bacterial Proteins
/
Transformation, Bacterial
/
Bacteriophages
/
Virology
/
DNA Restriction-Modification Enzymes
/
Corynebacterium
/
Galectins
/
Corynebacterium glutamicum
/
Fermentation
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS