Therapeutic efficacy of bone marrow-derived mesenchymal stem cells infused into mice with liver injury induced by concanavalin A / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1289-1293, 2009.
Article
in Zh
| WPRIM
| ID: wpr-343300
Responsible library:
WPRO
ABSTRACT
The aim of this study was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells (MSC) on acute liver injury induced by concanavalin A (ConA). MSCs were isolated from male C57BL/6 mice and cultured, and a ConA-induced acute liver injury model was used. MSCs were systemically infused immediately after mice were challenged with ConA, control mice received only saline infusion. 24 hours after MSC transplantation, the level of serum aminotransferases, histologic change and in situ apoptosis of cells were detected, the expression of inflammatory mediators were examined by real-time RT-PCR. The results indicated that MSC transplantation significantly reduced ConA-induced acute liver injury, including the decrease of the level of serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST) and the extenuation of liver necrosis and in situ apoptosis. Furthermore, after MSC infusion the expression of TNF-alpha, IFN-gamma in liver decreased greatly (p<0.05) with no statistical difference in the expression of iNOS, IL-2 and IL-10 (p>0.05). It is concluded that the systemic infusion of MSCs can alleviate ConA induced acute liver injury in mice.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Therapeutics
/
Bone Marrow Cells
/
Interferon-gamma
/
Interleukin-2
/
Tumor Necrosis Factor-alpha
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Treatment Outcome
/
Interleukin-10
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Concanavalin A
/
Cell Biology
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article