Organ-specific T cell receptor repertoire in target organs of murine graft-versus-host disease after haploidentical bone marrow transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 999-1004, 2009.
Article
in Zh
| WPRIM
| ID: wpr-343362
Responsible library:
WPRO
ABSTRACT
This study was purpused to analyze the characteristics of T cell receptor repertoire in target organs of murine graft-versus-host after haploidentical bone marrow transplantation (hiBMT) and the molecular characteristics of complementarity determining region3 (CDR3) repertoires of monoclonal T cell in liver, skin and ileum in murine after hiBMT. Murine haploidentical BMT model was established, CDR3-size spectratyping was used to study TCRBV repertoires in recipient liver, skin, ileum, spleen and a group of CDR3 molecules was obtained from GVHD-target tissues. The results showed that GVHD occurred as early as days 14 after transplantation and was proven by histology in liver, skin and ileum. A number of new monoclonal and oligoclonal T cells emerged in GVHD-target tissue. 45 CDR3 molecules had six C'-terminal motifs, which obtained from liver, skin, ileum in different times after hiBMT. It is concluded that target organs of murine graft-versus-host disease after hiBMT emerged a number of clonal or oligoclonal T cells, part of this T cell clones commonly uses some conserved CDR3 motifs and may recognize similar antigen.
Full text:
1
Index:
WPRIM
Main subject:
Immunoglobulin Variable Region
/
Molecular Sequence Data
/
Receptors, Antigen, T-Cell
/
Amino Acid Sequence
/
Bone Marrow Transplantation
/
Complementarity Determining Regions
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Allergy and Immunology
/
Genetics
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Graft vs Host Disease
/
Mice, Inbred BALB C
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article