Activated T cells promote differentiation of mesenchymal stem cells into osteoblasts / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 974-976, 2009.
Article
in Zh
| WPRIM
| ID: wpr-343368
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the effect of activated T cell on the ability of MSC to differentiate into osteoblasts. The activated T cells with MSCs were co-culture for 14 days, then the osteoblast formation was tested by alkaline phosphatase staining. Furthermore, the supernatant of activated T cell was added in culture system of MSCs, the expression of molecules related with immune regulation of activated T cells was detected by RT-PCR, so as to determine what kinds of cytokine displayed the important function in MSC differentiation. The result showed that activated T cell could promote differentiation of MSC into osteoblasts, and IL-1beta played an important role in the effect of activated T cells on MSCs, while TNF-alpha, TGF-beta1 were not. It is concluded that the activated T cells promote the differentiation of MSCs to osteoblasts. The interactive influence between MSCs and immune cells can be mediated through cytokines.
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
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T-Lymphocytes
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Cell Differentiation
/
Cells, Cultured
/
Culture Media, Conditioned
/
Coculture Techniques
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Cell Biology
/
Interleukin-1beta
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Mesenchymal Stem Cells
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article