Expression characteristics of mouse Catsper--the structural protein of sperm hyperactivation / 中华男科学杂志
National Journal of Andrology
; (12): 675-680, 2006.
Article
in Zh
| WPRIM
| ID: wpr-343548
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression specificity in tissues of the Catsper and its expression rules, which is a specification channels in sperm and plays an important role in sperm hyperactivation.</p><p><b>METHODS</b>The expression of Catsper in eight different tissues was examined by RT-PCR and immunohischemistry, the location of Catsper in sperm determined by immunofluorescence and the expression changes of Catsper analyzed in mice of different age groups.</p><p><b>RESULTS</b>Catsper was expressed specifically in the testis and epididymis, and was mainly located in the principal piece of the sperm tail. Catsper gene expression was developmentally regulated with a direct correlation between Catsper expression and mouse sexual maturation. Catsper gene experssion was first detected at 3 weeks of age and increased significantly till 6 weeks of age and then gradually to a stationary phase.</p><p><b>CONCLUSION</b>Catsper expresses in the testis and epididymis, and its growth period and expression amount are closely related with the developmental stages of the mouse.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Sperm Motility
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Spermatozoa
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Testis
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Calcium Channels
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Gene Expression
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Reverse Transcriptase Polymerase Chain Reaction
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Epididymis
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Genetics
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Metabolism
Limits:
Animals
Language:
Zh
Journal:
National Journal of Andrology
Year:
2006
Type:
Article