Paraquat involves differentiation of human neural stem cells via Notch signaling / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 492-495, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-343622
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of paraquat on the mRNA expression of key elements of Notch signaling (Notch1, Jagged1 and DTX1) during differentiation process of human neural stem cells (hNSCs).</p><p><b>METHODS</b>hNSCs exposed to PQ at the concentrations 0.10, 1.00, 10.00 M. Cell proliferation ability was assessed using MTT assay and mRNA expressions of Notch1, Jagged1 and DTX1 were detected by Real-time RT-PCR at 2, 4, 8, 12 d of differentiation.</p><p><b>RESULTS</b>Compared with control group, NOTCH1, JAG1 mRNA expression levels exposed to PQ at the concentration of 0.10 M significantly reduced at 2, 4, 8 d and significantly went up at 12d (P < 0.01). Compared with control group, NOTCH1, JAG1 and DTX1 mRNA expression levels exposed to PQ at the concentration of 10.00 M significantly reduced at 2, 8, 12 d (P < 0.01). PQ could down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the early stage of differentiation, then up-regulate Notch1 mRNA expression, and down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the end of differentiation.</p><p><b>CONCLUSION</b>Notch signaling pathway may be involved in differentiation of neural stem cell exposed to PQ.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Paraquat
/
Pharmacology
/
Calcium-Binding Proteins
/
Signal Transduction
/
Cell Differentiation
/
Cells, Cultured
/
Cell Biology
/
Intercellular Signaling Peptides and Proteins
/
Ubiquitin-Protein Ligases
/
Receptor, Notch1
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2013
Type:
Article
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