Influence of Qingdai compound on expression of bcr/abl and JWA in K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 809-811, 2005.
Article
in Zh
| WPRIM
| ID: wpr-343882
Responsible library:
WPRO
ABSTRACT
To study the effects of Qingdai compound on proliferation and apoptosis of K562 cells, as well as the expression of bcr/abl and JWA mRNA, K562 cells were treated in culture with different concentrations of Qingdai compound (2.5, 5, 7.5, 10 and 20 mg/ml) and harvested at 24 hours. Then morphological changes were observed by light microscopy (LM); expressions of bcr/abl and JWA were detected with semi-quantitative RT-PCR. The results showed that morphological changes were observed as the increment of the Qingdai compound concentration. Inhibition effects on proliferation and apoptosis in K562 cells were seen. A concentration-dependent decreases were found in bcr-abl and JWA mRNA expression of K562 cells. Qingdai compound partially inhibited proliferation and induced apoptosis of K562 cells. Expressions of both bcr/abl and JWA, which took part in cell proliferation and apoptosis, were down-regulated in a dose dependent manner. In conclusion, Qingdai compound can partially inhibit the expressions of bcr/abl and JWA genes in K562 cells, and the clinical effect of Qingdai compound on CML may be associated with apoptosis of leukemic cells.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Gene Expression Regulation, Neoplastic
/
Fusion Proteins, bcr-abl
/
Apoptosis
/
K562 Cells
/
Reverse Transcriptase Polymerase Chain Reaction
/
Intracellular Signaling Peptides and Proteins
/
Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article