16β-hydroxylation of 4-androstene-3,17-dione by Aspergillus niger / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1481-1485, 2014.
Article
in Zh
| WPRIM
| ID: wpr-345576
Responsible library:
WPRO
ABSTRACT
In order to discover the steroid biotransformation ability of filamentous fungus Aspergillus niger TCCC41650, we studied the fermentation of 4-androstene-3,17-dione with A. niger TCCC41650. The transformation product was purified, crystallized and determined as 16β-hydroxy-androst-4-ene-3,17-dione by X-ray single crystal diffraction method. The best fermentation condition was found to be pH 6.0, ethanol amount 2% with a substrate concentration of 1 per thousand, the transformation rate is 85.81% after 72 h. Based on the best of our knowledge, 16β-hydroxylation rarely occurs in microbial transformations of steroid. This study laid the foundation for the research of 16β-hydroxylation steroids
Full text:
1
Index:
WPRIM
Main subject:
Aspergillus niger
/
Industrial Microbiology
/
Biotransformation
/
Fermentation
/
Hydroxylation
/
Androstenedione
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article