Display cellulolytic enzymes on Saccharomyces cerevisiae cell surface by using Flo1p as an anchor protein for cellulosic ethanol production / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1401-1413, 2014.
Article
in Zh
| WPRIM
| ID: wpr-345584
Responsible library:
WPRO
ABSTRACT
In this study, we constructed a yeast consortium surface-display expression system by using Flo1 as an anchor protein. Endoglucanase II (EGII) and cellobiohydrolase II (CBHII) from Trichoderma reesei, and β3-glucosidase 1 (BGLI) from Aspergillus aculeatus were immobilized on Saccharomyces cerevisiae Y5. We constructed the cellulose-displaying expression yeast consortium (Y5/fEGII:Y5/fCBHII:Y5/fBGLI = 1:1:1) and investigated the enzymatic ability and ethanol fermentation. The displayed cellulolytic enzymes was stabile during the 96-h fermentation. The yeast consortium produced 0.77 g/L ethanol from 10 g/L phosphoric acid swollen cellulose (PASC) within 96 h. The yield (in grams of ethanol produced per gram of carbohydrate consumed) was 0.35 g/g, which correspond to 68.6% of the theoretical yield.
Full text:
1
Index:
WPRIM
Main subject:
Protein Binding
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Aspergillus
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Saccharomyces cerevisiae
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Trichoderma
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Cellulase
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Cellulose
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Saccharomyces cerevisiae Proteins
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Mannose-Binding Lectins
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Cellulose 1,4-beta-Cellobiosidase
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Ethanol
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article