Enzymatic production of α-ketoglutaric acid by L-glutamate oxidase from L-glutamic acid / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1318-1322, 2014.
Article
in Zh
| WPRIM
| ID: wpr-345592
Responsible library:
WPRO
ABSTRACT
We produced α-ketoglutaric acid (α-KG) from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase (LGOX). First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2+ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions, the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.
Full text:
1
Index:
WPRIM
Main subject:
Streptomyces
/
Glutamic Acid
/
Fermentation
/
Genetics
/
Amino Acid Oxidoreductases
/
Ketoglutaric Acids
/
Metabolism
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article