Application of micro-column gel cards assay for direct Coombs test in diagnosis of autoimmune hemolytic anemia / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinic value of micro-column gel cards direct Coombs test (gel test) in diagnosis of autoimmune hemolytic anemia (AIHA).</p><p><b>METHODS</b>Specimens of 128 suspected AIHA patients were performed direct Coombs test by conventional tube or gel cards. The results of the two tests were compared. The hemoglobin concentrations, proportions of reticulocyte, serum levels of bilirubin and free hemoglobin were detected simultaneously and compared in subgroups.</p><p><b>RESULTS</b>The positive detection rate of direct Coombs test performed by gel test or tube were 88.4% and 37.7%, respectively.</p><p><b>CONCLUSION</b>Compared with the tube tests, gel test assay is more sensitive, easy to perform and standardized in diagnosis of AIHA, and the gel card can be stored for a long time. The gel test is valuable for the diagnosis of AIHA.</p>