Role of transient receptor potential melastatin 8 channels in migraine mechanism in rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 515-519, 2015.
Article
in Zh
| WPRIM
| ID: wpr-346116
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of transient receptor potential melastatin 8 (TRPM8) channels in migraine mechanism in rats by measuring the changes in expression of TRPM8 in the trigeminal nerve of rats with migraine.</p><p><b>METHODS</b>Twenty male Sprague-Dawley rats were randomly and equally divided into a blank control group and a model group. Nitroglycerin (10 mg/kg) was injected subcutaneously in the back of the neck once a week for 5 weeks, to prepared a rat model of migraine without aura. Normal saline was injected subcutaneously instead of nitroglycerin in the control group. At 4 hours after the final injection, behavior scoring of all rats was performed, and then the trigeminal nerve ganglions of rats in both groups were collected for measurement of expression of N-methyl-D-aspartate receptor (NMDAR), protein kinase A (PKA), and TRPM8 using immunohistochemical staining, immunofluorescence, and Western blot, respectively.</p><p><b>RESULTS</b>The behavior score in each week during the rat model preparing was significantly higher in the model group than in the control group (P<0.05). The expression of NMDAR, PKA, and TRPM8 in the model group was significantly higher than in the control group (P<0.01). Both the behavior score and the expression of NMDAR were positively correlated with the expression of TRPM8 (r=0.822 and 0.794 respectively; P<0.01).</p><p><b>CONCLUSIONS</b>TRPM8 may be involved in migraine mechanism probably by activation of the NMDAR pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Rats, Sprague-Dawley
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Receptors, N-Methyl-D-Aspartate
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Cyclic AMP-Dependent Protein Kinases
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TRPM Cation Channels
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Migraine Disorders
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2015
Type:
Article