The mechanisms of p21WAF1/Cip-1 expression in MOLT-4 cell line induced by TSA / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 174-181, 2005.
Article
in Zh
| WPRIM
| ID: wpr-347801
Responsible library:
WPRO
ABSTRACT
To investigate the function and molecular mechanism of p21(WAF1/Cip-1) expression in MOLT-4 cells induced by HDAC inhibitor TSA, the expression pattern of p21(WAF1/Cip-1) and the distribution of cell cycle in TSA treated cells were analyzed. The results showed that TSA could effectively induce G(2)/M arrest and apoptosis of MOLT-4 cells. Kinetic experiments demonstrated that p21(WAF1/Cip-1) were upregulated quickly before cell arrested in G(2)/M and began decreasing at the early stage of apoptosis. Meanwhile, the proteasome inhibitor MG-132 could inhibit the decrease of p21(WAF1/Cip-1) at the early stage of apoptosis, which showed that proteasome pathway involved in p21(WAF1/Cip-1) degradation during the TSA induced G(2)/M arrest and apoptosis responses. This study also identified that the protein level of p21(WAF1/Cip-1) was highly associated with the cell cycle change induced by TSA. Compared to cells treated by TSA only, exposure MOLT-4 cells to TSA meanwhile treatment with MG-132 increased the protein level of p21(WAF1/Cip-1) and increased the numbers of cell in G(2)/M-phase, whereas the cell apoptosis were delayed. It is concluded that p21(WAF1/Cip-1) plays a significant role in G(2)/M arrest and apoptosis signaling induced by TSA in MOLT-4 cells.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Leukemia, Myeloid
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Cell Cycle
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Blotting, Western
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Apoptosis
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Cell Line, Tumor
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Enzyme Inhibitors
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Cyclin-Dependent Kinase Inhibitor p21
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Histone Deacetylase Inhibitors
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article