Estrogenic activity and its mechanism of ethanol extract from black soybean / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 825-828, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-351782
ABSTRACT
<p><b>OBJECTIVE</b>To explore the estrogenic activity and its mechanism of ethanol extract from black soybean (BSE).</p><p><b>METHOD</b>The modified MCF-7 cell proliferation assay was used to evaluate the estrogenic activity of BSE. And the possible mechanisms were addressed using RT-PCR measurements in which pure estrogen receptor antagonist ICI182,780 was employed as a tool.</p><p><b>RESULT</b>The estrogenic activity of BSE at various concentrations (1-1000 microg x mL(-1)), expressed as proliferative effect (PE) relative to that of solvent control, was examined. The results indicated that, at low concentration range (10-200 microg x mL(-1)), BSE was able to induce MCF-7 cell growth with a maximum at 100 microg x mL(-1). The results of RT-PCR showed that pS2 and PR mRNA could be significantly induced by BSE in MCF-7 cells, which was relative to the concentration of BSE. Moreover, the specific estrogen receptor antagonist, ICI182,780 could block these reactions.</p><p><b>CONCLUSION</b>Ethanol extract of black soybean can stimulate the growth of MCF-7 cells and increase the expression of estrogen receptor-responsive gene, suggesting that the estrogenic effects of BSE are mediated by the estrogen receptor.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Plants, Medicinal
/
Seeds
/
Glycine max
/
Breast Neoplasms
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Receptors, Progesterone
/
Chemistry
Limits:
Female
/
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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