The preparation and bioactivity assessment of gene recombinant fibrin-binding brain derived neurotrophic factor / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1058-1062, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-352114
ABSTRACT
Using the E. coli, we fabricated the gene reconstructed brain derived neurotrophic factor with a fibrin binding domain (FBD-BDNF). We then tested the neurotrophic bioactivity and fibrin-binding ability of the FBD-BDNF. The E. coli was used to express the recombinant protein. The inclusion body was purified with column chromatography and renaturated to construct the right 3D formation. In this study, we successfully fabricated the FBD-BDNF and tested the binding ability and neurotrophic activity. The results demonstrated that FBD-BDNF had special binding ability of fibrin and significant neurotrophic activity for DRG cells. FBD-BDNF could have a promising application prospect in nerve tissue engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Recombinant Fusion Proteins
/
Fibrin
/
Brain-Derived Neurotrophic Factor
/
Escherichia coli
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS