Construction of Runx1 transgenic mice / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 584-587, 2013.
Article
in Zh
| WPRIM
| ID: wpr-352205
Responsible library:
WPRO
ABSTRACT
This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3. 1 Flag Runx1 FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection technology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runx1 transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.
Full text:
1
Index:
WPRIM
Main subject:
Recombination, Genetic
/
Mice, Transgenic
/
Molecular Sequence Data
/
Base Sequence
/
Core Binding Factor Alpha 2 Subunit
/
Genetic Vectors
/
Genetics
/
Mice, Inbred C57BL
/
Microinjections
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Journal of Biomedical Engineering
Year:
2013
Type:
Article