Inhibitory effect of cyclopamine on metastatic ability of EC109 cells and its mechanism / 南方医科大学学报
Journal of Southern Medical University
; (12): 1828-1832, 2012.
Article
in Zh
| WPRIM
| ID: wpr-352324
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cyclopamine on metastatic ability of human esophageal cancer EC109 cells and explore the possible mechanism.</p><p><b>METHODS</b>Transwell chamber assay and angiogenesis assay were used to examine the metastatic ability, invasiveness and angiogenesis of EC109 cells treated with cyclopamine for 48 h. The expression of Gli-1 mRNA was detected using RT-PCR, and Western blotting was used to examine the protein expressions of Gli-1, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF).</p><p><b>RESULTS</b>Inhibition of the hedgehog signaling pathway by cyclopamine suppressed the migration, invasion, and angiogenesis of EC109 cells. Cyclopamine treatment significantly lowered the expression of Gli-1 mRNA (P<0.05) and the protein expressions of Gli-1, MMP-9 and VEGF (P<0.05).</p><p><b>CONCLUSION</b>Cyclopamine can significantly inhibit the metastatic capacity of EC109 cells possibly by down-regulating MMP-9 and VEGF expression as a result of Gli-1 inhibition.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Transcription Factors
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Veratrum Alkaloids
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RNA, Messenger
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Esophageal Neoplasms
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Signal Transduction
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Gene Expression Regulation, Neoplastic
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Matrix Metalloproteinase 9
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Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article