Establishment of an isolated porcine liver machine perfusion model for high-intensity focused ultrasound studies / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of establishing an isolated porcine liver machine perfusion model and assess its value in high-intensity focused ultrasound studies.</p><p><b>METHODS</b>Twenty-one isolated porcine livers were perfused with autologous blood for 4 h through dual vessels (portal vein and hepatic artery) cannulation using an extracorporeal circulation machine under a sub-normothermic perfusion condition. The perfusion model was assessed by monitoring the liver color, texture, liver weight gain, hemodynamic parameters, color Doppler flow imaging, bile output and histopathology.</p><p><b>RESULTS</b>Nineteen isolated porcine livers were successfully cannulated with dual vessels, and failure of hepatic artery intubation occurred in two porcine livers. After machine perfusion for 4 h, the isolated livers maintained a soft texture with stable hemodynamic levels within relative normal physiological ranges. The bile output was more than 3 ml/h within the initial 3 h of perfusion. Histopathological examination demonstrated no morphological or structural changes of the liver tissues.</p><p><b>CONCLUSION</b>The isolated porcine liver perfusion model is stable and feasible, and can be used for high-intensity focused ultrasound studies.</p>