SNCG shRNA suppressed breast cancer cell xenograft formation and growth in nude mice / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1524-1528, 2011.
Article
in En
| WPRIM
| ID: wpr-353951
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Overexpression of breast cancer-specific gene 1 (SNCG) is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA (shRNA).</p><p><b>METHODS</b>Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors. These vectors were then stably transfected into a breast cancer MCF-7 cell line to knockdown SNCG expression. After SNCG knockdown was confirmed, the stable cell lines were inoculated into nude mice. SNCG mRNA and protein expressions were analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively in both the stable cell lines and xenografts.</p><p><b>RESULTS</b>All four SNCG shRNA constructs significantly reduced SNCG mRNA and protein levels in MCF-7 cells, as compared to the unrelated sequence control shRNA and the liposome control mice (P < 0.05). SNCG-knockdown MCF-7 cells formed significantly smaller tumor masses than cells expressing the unrelated sequence control or the liposome control mice (P < 0.05).</p><p><b>CONCLUSION</b>SNCG shRNA effectively suppressed breast cancer cell formation in vivo and may be a useful clinical strategy to control breast cancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
/
Therapeutics
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Breast Neoplasms
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Immunohistochemistry
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Gene Expression Regulation, Neoplastic
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Reverse Transcriptase Polymerase Chain Reaction
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Xenograft Model Antitumor Assays
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RNA, Small Interfering
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Cell Line, Tumor
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Gamma-Synuclein
Limits:
Animals
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Female
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Humans
Language:
En
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article