Effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1884-1888, 2011.
Article
in Zh
| WPRIM
| ID: wpr-354168
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.</p><p><b>METHOD</b>The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.</p><p><b>RESULT</b>The QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).</p><p><b>CONCLUSION</b>QKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Complement System Proteins
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Drugs, Chinese Herbal
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Cell Degranulation
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Chemistry
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Chemistry, Pharmaceutical
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Bodily Secretions
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Cell Line, Tumor
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Hexosaminidases
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Histamine Release
Limits:
Animals
/
Humans
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article