Comparative proteomics research of apoptosis initiation induced by homoharringtonine in HL-60 cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 624-628, 2003.
Article
in Zh
| WPRIM
| ID: wpr-354815
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the related proteins of apoptosis initiation induced by homoharringtonine (HHT) in HL-60 cells.</p><p><b>METHODS</b>After establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.</p><p><b>RESULTS</b>Proteomics analysis showed that proteins including MHC class I antigen, calbindin D-28K, chloride channel protein 6, oncoprotein 18, zinc finger protein Helios and apoptosis inhibitor like protein 2 were involved in apoptosis initiation induced by HHT.</p><p><b>CONCLUSION</b>The present study might conduce to the researches of HL-60 cells carcinogenesis and pave the way to exploit drug precursor related to HHT and initiation of apoptosis in HL-60 cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Phosphoproteins
/
Transcription Factors
/
S100 Calcium Binding Protein G
/
Electrophoresis, Gel, Two-Dimensional
/
Histocompatibility Antigens Class I
/
Proteins
/
Apoptosis
/
Chloride Channels
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2003
Type:
Article